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51314-3757-Testing Continuous Biocide Injection in a Seawater Injection Scenario

Picture for Testing Continuous Biocide Injection in a Seawater Injection Scenario
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Product Number: 51314-3757-SG
ISBN: 3757 2014 CP
Author: Jan Larsen
Publication Date: 2014
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Injection water is typically batch treated with biocides to minimize biofouling of water injection systems. This paper describes a study with biofilm reactors to determine the biocidal effect of TetrakisHydroxyMethylPhosphonium Sulfate (THPS). The results demonstrate how operators can optimize their use of biocides by testing the effect of different treatment conditions including concentrations exposure time and continuous versus batch treatment on biofilm from oil field systems.The tests were conducted using injection seawater and biofilm from the Dan field in the Danish Sector of the North Sea. Four anaerobic biofilm reactors were operated in parallel to compare the effect of 30 ppm and 60 ppm continuous THPS product treatment and a two hours 800 ppm batch treatment to an untreated control. Biofilm activity was determined by Reverse Transcriptase qPCR (RT-qPCR) sulfate reduction rates (SRR) using radiolabeled 35SO42- and measurements of metabolites.Sulfate-reducing Bacteria (SRB) were active in all four biofilm reactors at the end of the experimental period. Continuous biocide treatment with 30 ppm and 60 ppm THPS product reduced SRR by a factor 2 and 4 compared to the untreated control. Sulfate reduction was only temporarily inhibited by the 800 ppm batch treatment as activity recovered after 1½ days.Keywords: TetrakisHydroxyMethylPhosphonium Sulfate (THPS) molecular microbiological methods (MMM) Reverse Transcriptase qPCR (RT-qPCR) sulfate reduction rates (SRR) sulfate-reducing Bacteria (SRB) biofilm Dan field Danish Sector of the North Sea.
Injection water is typically batch treated with biocides to minimize biofouling of water injection systems. This paper describes a study with biofilm reactors to determine the biocidal effect of TetrakisHydroxyMethylPhosphonium Sulfate (THPS). The results demonstrate how operators can optimize their use of biocides by testing the effect of different treatment conditions including concentrations exposure time and continuous versus batch treatment on biofilm from oil field systems.The tests were conducted using injection seawater and biofilm from the Dan field in the Danish Sector of the North Sea. Four anaerobic biofilm reactors were operated in parallel to compare the effect of 30 ppm and 60 ppm continuous THPS product treatment and a two hours 800 ppm batch treatment to an untreated control. Biofilm activity was determined by Reverse Transcriptase qPCR (RT-qPCR) sulfate reduction rates (SRR) using radiolabeled 35SO42- and measurements of metabolites.Sulfate-reducing Bacteria (SRB) were active in all four biofilm reactors at the end of the experimental period. Continuous biocide treatment with 30 ppm and 60 ppm THPS product reduced SRR by a factor 2 and 4 compared to the untreated control. Sulfate reduction was only temporarily inhibited by the 800 ppm batch treatment as activity recovered after 1½ days.Keywords: TetrakisHydroxyMethylPhosphonium Sulfate (THPS) molecular microbiological methods (MMM) Reverse Transcriptase qPCR (RT-qPCR) sulfate reduction rates (SRR) sulfate-reducing Bacteria (SRB) biofilm Dan field Danish Sector of the North Sea.
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