The efficiency of biocides can be improved by optimizing treatment programs and including the inhibiting effect of other production chemicals. Here the efficiency of batch versus continuous treatment with a biocide formulation of TetrakisHydroxyMethylPhosphonium Sulfate (THPS) was investigated in a setup with flow-through bioreactors containing biofilm derived from the Danish Sector of the North Sea. The experiments were performed with injection seawater (ISW) produced water (PW) and amended with spent H2S scavenger (SS) and scale inhibitor (SI). In 100% ISW sulfate-reducing Bacteria (SRB) regained their activity within 2 days after 800 ppm batch treatment and continuous 60 ppm dosage of biocide decreased the SRB activity by approximately 60%. Presence of SS and SI had a significant biocidal effect in 100% ISW and eliminated the need for biocide. Results were different in bioreactors with PW. In a 30:70 mixture of ISW and PW with SS and SI SS and SI had little biocidal effect whereas both continuous and batch biocide dosing reduced biofilm activity. In contrast to the ISW scenario it took more than 5 days for the SRB to recover after the 800 ppm batch THPS treatment. In 100% PW with SS and SI the continuous biocide treatment had no significant effect and batch dosage only decreased microbial activity slightly. These studies illustrate how biocide treatment programs can be optimized depending on the water source.