Recent field reports have suggested that microbiologically influenced corrosion (MIC) is occurring at biologically favorable sites in equipment handling ethanol fuels. Interstate pipeline transportation of ethanol fuels could increase the potential consequence of an MIC related failure. Microbial communities in ethanol spillage and runoff tanks at a fueling terminal were indentified using Sanger sequencing of ribosomal rRNA genes. Microbes were cultivated from the samples for a laboratory MIC study. MIC of API?5L X52 and X70 steels was evaluated using electrochemical impedance spectroscopy (EIS) and electron microscopy. Acid production bacteria (APB) associated with a high tendency for pitting. Based on experimental observations it is theorized that pitting can occur by two mechanisms: (1) Acetic acid-enhanced corrosion driven by galvanic coupling between the ferrite matrix and second phase particles (2) Local colonization by APB cells. Sulfate reducing bacteria (SRB) enhanced general corrosion and also caused pitting and etch-pitting. Corrosion behavior is believed to be controlled by the evolution of the iron sulfide corrosion product layer. The integrity of this film is observed to be dependent on the presence of acetic acid and microbial colonization of the film. This laboratory investigation along with recent field reports demonstrates and describes a threat of MIC at sites in ethanol transportation systems where conditions are biologically suitable. Recommendations are made for prevention and remediation.