51316-7793-Integration of Electron Impedance Spectroscopy and Microfluidics for Investigating MIC Using Co-Culture Biofilms

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Product Number: 51316-7793-SG

ISBN: 7793 2016 CP

Author: Susmitha Purnima Kotu

Publication Date: 2016

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Microbially influenced corrosion (MIC) is a major problem in various sectors including chemical process plants on-shore and off-shore oil and gas pipelines marine and aviation industries. Annual losses associated with MIC in the United States are estimated to be $50 billion. MIC often develops as a result of biofilm formation by multiple microbial species that form well-defined and organized structures. Development of effective mitigation strategies for MIC requires a fundamental understanding of how biofilms are formed. The aim of this study is to investigate the factors underlying formation and development of dual-culture biofilms. UsingShewanella oneidensis as the model aerobic biofilm forming species and Desulfovibrio vulgaris as the model anaerobe and sulfate reducing bacterium we investigated the dynamics of biofilm formation using a microfluidic flow cell. The experimental system consists of a flow channel in a microfluidic device made of polydimethylsiloxane that is bonded to a glass slide with coated metal electrodes. Co-culture biofilms ofS. oneidensis and D. vulgaris developed in this system were used to develop a correlation between the thickness of biofilm as measured by confocal laser scanning microscope (CLSM) and impedance measurement from electron impedance spectroscopy (EIS). The effect of hydrodynamic factors like flow rate shear stress on biofilm dynamics was also investigated. In addition the effect of various typical biocides on these co-culture biofilms was studied with respect to biocide penetration efficacy of the kill and biofilm regrowth.Keywords: Confocal laser scanning microscope (CLSM) Electron impedance spectroscopy (EIS) Microfluidic devices Co-culture biofilms