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51316-7770-Adding it All Up - The Fate of Sessile Microbes Following Biocide Treatment.

Product Number: 51316-7770-SG
ISBN: 7770 2016 CP
Author: Timothy Tidwell
Publication Date: 2016
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Microbially Influenced Corrosion is primarily caused by sessile microbes found within a biofilm. The ultimate goal of many biocide treatments is the removal of any biofilm within that system. These cells may be killed but remain in place be removed but not killed or may be both killed and removed. Many of the factors that determine their fate are poorly understood. For example deciding to treat a system with a low dose of biocide for a long period of time or to treat with a high dose of biocide for a short period of time may yield drastically different results in terms of reducing the risk of microbially influenced corrosion. In this study we show the kinetics of biofilm removal using oilfield biofilms treated in a flow cell along with confocal microscopy. Additionally we also show how analysis of the bulk fluid following a biocide treatment with flow cytometry and ATP quantification can be used to better understand the kinetics of biofilm removal and the ultimate fate of the microbes that resided therein.
Microbially Influenced Corrosion is primarily caused by sessile microbes found within a biofilm. The ultimate goal of many biocide treatments is the removal of any biofilm within that system. These cells may be killed but remain in place be removed but not killed or may be both killed and removed. Many of the factors that determine their fate are poorly understood. For example deciding to treat a system with a low dose of biocide for a long period of time or to treat with a high dose of biocide for a short period of time may yield drastically different results in terms of reducing the risk of microbially influenced corrosion. In this study we show the kinetics of biofilm removal using oilfield biofilms treated in a flow cell along with confocal microscopy. Additionally we also show how analysis of the bulk fluid following a biocide treatment with flow cytometry and ATP quantification can be used to better understand the kinetics of biofilm removal and the ultimate fate of the microbes that resided therein.
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