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Expanding Industry Access to Molecular Microbiological Methods: Development of an Off-the-Shelf Laboratory Workflow for qPCR and NGS Analysis

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Multiple families of microorganisms have been implicated in Microbiologically Influenced Corrosion (MIC) within the oil and gas industry as well as in other fields resulting in billions of dollars in damage annually. The ability to detect these organisms in a timely and accurate manner can allow for the implementation of appropriate treatment regimens that mitigate costs and minimize downtime.Molecular Microbiological Methods (MMMs) such as quantitative polymerase chain reaction (qPCR) and next-generation sequencing (NGS) can provide unparalleled insight into microbial communities for diagnosing and managing MIC. However the routine application of MMMs by industry is still limited today due to high testing costs and access to only a relatively small number of commercial or internal company laboratories with MMM testing capabilities.Our recent work has focused on building a complete off-the-shelf laboratory workflow for conducting qPCR and NGS-based 16S rRNA metagenomic analysis. This workflow includes: (1) easy-to-use kits for sample collection field sample preservation and nucleic acid extraction (2) a pre-optimized panel of qPCR assays targeting important MIC-related microorganisms such as Sulfate-Reducing Prokaryotes (SRPs) and (3) access to a secure online portal for automated processing and analysis of NGS data. The availability of this workflow will allow virtually any testing laboratory with moderately skilled personnel to quickly and confidently begin conducting MMM-based analyses. We report results encompassing the development and initial validation of this workflow using samples collected from oil and gas and wastewater facilities. This new workflow will significantly reduce testing costs help to standardize testing across laboratories and provide significantly greater industry access to these powerful analysis technologies.

Product Number: 51319-13033-SG
Author: Neil Sharma
Publication Date: 2019
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