51316-7698-Molecular Techniques for onsite monitoring of microbial populations in oilfields – Overview Perspectives and Evaluation

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Product Number: 51316-7698-SG

ISBN: 7698 2016 CP

Author: Renato De Paula

Publication Date: 2016

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Microorganisms can pose serious challenges to oil and gas operations by generating biotic H2S accelerating localized corrosion and causing fouling of equipment. Proper monitoring of microbes in the field is a crucial step in any mitigation program and the selection of the test methods is critical to achieve meaningful accurate and timely results. Determination of microbial counts can be accomplished by many techniques including traditional culture-based and advanced molecular methods which require significant laboratory and technical capabilities. Nevertheless molecular methods such as qPCR and microbial identification using Next Generation DNA Sequencing have become increasingly accepted by the industry. However many challenges remain in regards to using molecular techniques for routine monitoring in the fields. These include sample preservation shipping logistics turnaround time and costs. These challenges have prevented a complete acceptance of molecular methods into oil and gas applications. This study describes the evaluation of technologies that allow historically lab based methods to move into the field while overcoming the most common field barriers. The end result is the development of molecular methods for use in the field that can be performed in a simple and quick way.