Server maintenance is scheduled for Saturday, December 21st between 6am-10am CST.

During that time, parts of our website will be affected until maintenance is completed. Thank you for your patience.

Menu
Search
Filters
Close
Menu

51318-11662-DNA sequencing of oilfield samples: impact of protocol choices on the microbiological conclusions

Picture for DNA sequencing of oilfield samples: impact of protocol choices on the microbiological conclusions
Available for download

In this study, we retrieved multiple samples from several wells in an onshore oilfield and submitted them for 16S rDNA taxonomic analysis in two different laboratories. The results showed significant differences between laboratories.

 

Product Number: 51318-11662-SG
Author: Renato De Paula / Cruz St Peter / Ian Alex Richardson / Jep Bracey / Ed Heaver / Kathleen Duncan / Mary Eid / Ralph Tanner
Publication Date: 2018
Industries: Test Method , Corrosion Monitoring and Control
$0.00
$20.00
$20.00

In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and separation facilities. Nonetheless, many molecular procedures cannot be performed onsite and samples are typically sent offsite for specialized analyses. The lack of standard procedures hinders comparison of findings between laboratories. Operators currently use dissimilar sampling and preservation protocols, different methods for DNA extraction, separate sequencing platforms and varied approaches for the analyses of the resulting molecular data. In this study, we retrieved multiple samples from several wells in an onshore oilfield and submitted them for 16S rDNA taxonomic analysis in two different laboratories. The results showed significant differences between laboratories in the total abundance of organisms, their taxonomic composition and the presence/absence of certain diagnostic bacteria. Close examination of the protocols revealed that the sample preservation techniques and specific 16S rDNA gene primer sets likely had a significant impact on the resulting information. Collectively, this experience suggests that while molecular techniques are extremely powerful tools to analyze oilfield microbiology, the lack of consensus on an industry wide protocol may lead to discrepancies that could negatively impact the exploitation of these promising methods.

Key words: Microorganisms, qPCR, NGS, detection, molecular techniques, monitoring

In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and separation facilities. Nonetheless, many molecular procedures cannot be performed onsite and samples are typically sent offsite for specialized analyses. The lack of standard procedures hinders comparison of findings between laboratories. Operators currently use dissimilar sampling and preservation protocols, different methods for DNA extraction, separate sequencing platforms and varied approaches for the analyses of the resulting molecular data. In this study, we retrieved multiple samples from several wells in an onshore oilfield and submitted them for 16S rDNA taxonomic analysis in two different laboratories. The results showed significant differences between laboratories in the total abundance of organisms, their taxonomic composition and the presence/absence of certain diagnostic bacteria. Close examination of the protocols revealed that the sample preservation techniques and specific 16S rDNA gene primer sets likely had a significant impact on the resulting information. Collectively, this experience suggests that while molecular techniques are extremely powerful tools to analyze oilfield microbiology, the lack of consensus on an industry wide protocol may lead to discrepancies that could negatively impact the exploitation of these promising methods.

Key words: Microorganisms, qPCR, NGS, detection, molecular techniques, monitoring

Product tags
Also Purchased
Picture for Corrosion Inhibitor Screening - Impact of Test Approaches & Conditions on Performance
Available for download

51318-11654-Corrosion Inhibitor Screening - Impact of Test Approaches & Conditions on Performance

Product Number: 51318-11654-SG
Author: Ian Carpenter / Hunter Thomson / Rodney Madjitey / Caroline M.E. Simpson
Publication Date: 2018
$20.00

This paper provides illustrative examples of how seemingly small changes to test methodology in preliminary screening tests can significantly influence the absolute performance and relative ranking of corrosion inhibitors.
Picture for Practical Cost-Benefit Analysis For Pipeline Corrosion Management
Available for download

51318-11641-Practical Cost-Benefit Analysis For Pipeline Corrosion Management

Product Number: 51318-11641-SG
Author: Tony Alfano / Tom Yahner / Dr. William V Harper / Jeff Kobs
Publication Date: 2018
$20.00

Framework for the management of corrosion life-cycle costs including the assessment of risk over time, the establishment of statistical process control techniques for identifying and evaluating risk decisions - and the methodology.
Picture for A condition monitor for atmospheric induced stress corrosion cracking
Available for download

51318-11634-A condition monitor for atmospheric induced stress corrosion cracking

Product Number: 51318-11634-SG
Author: A Condition Monitor for Atmospheric Induced Stress Corrosion Cracking
Publication Date: 2018
$20.00

A sacrificial sensor has been designed to detect the occurrence and rate of atmospheric corrosion and stress corrosion cracking. Constructed using additive manufacturing and can be customized for use with small size wires or ribbons of any metallic material as a sensor electrode.
Categories
Industry
Recently viewed
Popular tags
View all

Association for Materials Protection and Performance

© AMPP All Rights Reserved.
Membership Terms of Content Use
Contact Customer Support