Menu
Search
Filters
Close
Menu

51315-5555-Direct Comparison of Microbial Population Analysis Using ATP Quantification Culture Based Enumeration in Bug Bottles Epifluorescence Microscopy and Shotgun Population Analysis by Sequencing 16S Amplicons

  • /
  • /
  • /
  • 51315-5555-Direct Comparison of Microbial Population Analysis Using ATP Quantification Culture Based Enumeration in Bug Bottles Epifluorescence Microscopy and Shotgun Population Analysis by Sequencing 16S Amplicons
Picture for Direct Comparison of Microbial Population Analysis Using ATP Quantification Culture Based Enumerati
Available for download
Product Number: 51315-5555-SG
ISBN: 5555 2015 CP
Author: Jennifer Fichter
Publication Date: 2015
$0.00
$20.00
$20.00

Monitoring of microbial populations in oil and gas operations is routinely conducted in order to evaluate the need for and performance of biocides. Operators utilize a variety of methods designed to determine if bacteria and archaea capable of causing corrosion fouling or souring are present in the systems before during and after control methods are applied. Each monitoring method has strengths and limitations and an understanding of the strengths and limitations is crucial to deciding the best method for any given system. The most obvious considerations are the accuracy of the test cost ease of use and time required to obtain the results. How comparable results are from a given location or time to another time and the value of the data for making higher level decisions also should be considered. In this study we will share the results obtained from analyzing samples collected from various phases of natural gas and oil production operations. These locations include microbial populations collected from a range of operating temperatures pressures and salinities as well as those treated with different types of biocides. Four methods were directly compared: 1.) culture based enumeration using bug bottles with indicator media for SRB APB and IRB 2.) the rapid ATP quantification assay 3.) epifluorescence microscopy and 4.) molecular analysis of the microbial populations by DNA isolation and sequencing of 16s amplicons. The results are discussed in terms of agreement between the methods difficulty of the approaches and the relative value of each data set for future use.

Monitoring of microbial populations in oil and gas operations is routinely conducted in order to evaluate the need for and performance of biocides. Operators utilize a variety of methods designed to determine if bacteria and archaea capable of causing corrosion fouling or souring are present in the systems before during and after control methods are applied. Each monitoring method has strengths and limitations and an understanding of the strengths and limitations is crucial to deciding the best method for any given system. The most obvious considerations are the accuracy of the test cost ease of use and time required to obtain the results. How comparable results are from a given location or time to another time and the value of the data for making higher level decisions also should be considered. In this study we will share the results obtained from analyzing samples collected from various phases of natural gas and oil production operations. These locations include microbial populations collected from a range of operating temperatures pressures and salinities as well as those treated with different types of biocides. Four methods were directly compared: 1.) culture based enumeration using bug bottles with indicator media for SRB APB and IRB 2.) the rapid ATP quantification assay 3.) epifluorescence microscopy and 4.) molecular analysis of the microbial populations by DNA isolation and sequencing of 16s amplicons. The results are discussed in terms of agreement between the methods difficulty of the approaches and the relative value of each data set for future use.

Also Purchased
Picture for How Hot is Too Hot for Bacteria? A Technical Study Assessing Bacterial Establishment in Downhole Dri
Available for download

51312-01310-How Hot is Too Hot for Bacteria? A Technical Study Assessing Bacterial Establishment in Downhole Dri

Product Number: 51312-01310-SG
ISBN: 01310 2012 CP
Author: Jennifer Fichter
Publication Date: 2012
$20.00
Picture for 10404 Protection Against Microbiologically Influenced Corrosion by Effective Treatment
Available for download

10404 Protection Against Microbiologically Influenced Corrosion by Effective Treatment and Monitoring During Hydrotest Shut-In

Product Number: 51300-10404-SG
ISBN: 10404 2010 CP
Author: Joseph E. Penkala, Jennifer Fichter, and
Publication Date: 2010
$20.00
Picture for 08658 Use of Microbiocides in Barnett Shale Gas Well Fracturing Fluids to Control Bacterially Relate
Available for download

08658 Use of Microbiocides in Barnett Shale Gas Well Fracturing Fluids to Control Bacterially Related Problems

Product Number: 51300-08658-SG
ISBN: 08658 2008 CP
Author: Jennifer K. Fichter, Kelly Johnson, Karl D. French, and Rod Owen
Publication Date: 2008
$20.00
Categories
Industry
Recently viewed
Popular tags
View all

Association for Materials Protection and Performance

© AMPP All Rights Reserved.
Membership Terms of Content Use
Contact Customer Support