Infrared Spectroscopy Technique based on diffuse, internal and external reflectance allows both, the
performance of a general qualitative analysis of the structure and the identification of organic and inorganic
compounds, through the excitation of molecular vibrations by light absorption. Through the technique,
vibrational data is obtained from the surface film, determining the nature of the molecules adsorbed on the
metallic surface. Different researchers have given their attention and efforts to the study of the interfase
region, by using Fourier Transformed Infrared Spectroscopy (FTIR) with Attenuated Total Reflectance
(ATR). These efforts have mainly evaluated the Extracellular Polymeric Substances (EPS) and their
contribution to corrosion. In order to improve the understanding of the role of bacterial biofilm formed
during Microbiologically Influenced Corrosion (MIC) processes, in situ FTIR was used to study changes on
the surface under the biofilm and analyze the effect of bacterial activity. In this study, FTIR was used in
conjunction with a potentiostat/galvanostat coupled to a newly developed electrochemical cell to follow up
the reactions generated during MIC processes by Sulphate Reducing Bacteria (SRB).