07530 Comparison of Bacterial Growth of Sulfate Reducing Bacteria Evaluated by Serial Dilution, Pure Plate and Epifluorescence Techniques

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Product Number: 51300-07530-SG

ISBN: 07530 2007 CP

Author: M. Romero, L. Ocando, M. Garcia, M. Bracho, M. Sarro, S. Borgne, and I. Araujo

Publication Date: 2007

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In order to confirm Microbiologically Influenced Corrosion (MIC), it is essential to document the presence of potentially involved microorganisms. However, the total number of bacteria present in a biofilm is seldom estimated because it contains a large bacterial population in addition to the fact that viable but non-cultivable (VNC) cells cannot be recovered by traditional enumeration technique such as the spread plate and the multiple tube methods. A spectrum of alternative methodological approaches is now either under development or available to detect and quantify sulfate reducing bacteria (SRB) or their activities in laboratories and environmental studies. A variety of microscopic methods have been successfully adapted to such studies including bright-field microcopy and more recently, epifluorescence and confocal laser microscopy, which are more sensitive but also more expensive and require a degree of expertise not normally encountered outside the specialist laboratory. In this work, total count, viable and VNC SRB were counted using traditional microbiological culture-based techniques and epifluorescence microscopy with special fluorescent dyes for both dead and live bacteria, in order to find a correlation between these methods potentially useful for industrial purposes.