07523 Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry

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Product Number: 51300-07523-SG

ISBN: 07523 2007 CP

Author: Sylvie Le Borgne, Hector A. Videla, Juan Manuel Romero, Juan M. Gonzalez, and Cesareo Saiz-Jimenez

Publication Date: 2007

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Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitoring of the system showed that persistent bacteria were present, mainly facultative anaerobic bacteria as Shewanella and Vibrio. These bacteria are also suspected to resist the biocide treatment used in this system. A second case was based on a study of wastewater treatment plants where massive sulfide production generally occurs during organic matter mineralization.