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01260 HYDROGEN PERMEATION STUDY WITH PALLADIUM IN A SULFATE-REDUCING BACTERIA CULTURE

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Product Number: 51300-01260-SG
ISBN: 01260 2001 CP
Author: Matilde F. de Romero, Zoilabet Duque C., Oladis T. de Rincon, Orlando Perez Ismenia Araujo
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This study was undertaken to evaluate cathodic depolarization as the action mechanism triggered by sulfate-reducing bacterias (SRBs) in Microbiologically Induced Corrosion (MIC), using an inert substrate such as a l-ram thick Palladium (Pd) strip with and without cathodic polarization, a H ° permeation cell type by Devanathan and Stachurski, and the bacteria Desulfovibrio desuifuricans ssp. desulfuricans. The permeation tests were run using a de-aerated sterile culture medium inoculated with 10% D. dexulfuricans at 108 cell/ml. Bacterial growth was evaluated by the serial dilution technique and Scanning Electron Microscopy (SEM) was used to analyze the characteristics of the biofilm formed on the Pd strip. Results indicated bacterial growth in the order of40xl09 CFU/ml at 24 hours in both polarization and non-polarization tests. This shows that these bacteria develop similarly, whether or not they are on a polarized surface as a source of H °, generating H2S as a product of sulfate-dissimilating activity. It was also determined that, without cathodic polarization, there were no conditions for reduction and permeation of the tf generated by H2S dissociation. When cathodic polarization was applied, an increase was observed in the permeation current, which was associated with the maximum enzymatic activity phase of the bacteria.

Key Words: Hydrogen permeation cell, cathodic depolarization mechanism, Sulfate- Reducing Bacteria, Microbiologically Induced Corrosion, cathodic polarization.
This study was undertaken to evaluate cathodic depolarization as the action mechanism triggered by sulfate-reducing bacterias (SRBs) in Microbiologically Induced Corrosion (MIC), using an inert substrate such as a l-ram thick Palladium (Pd) strip with and without cathodic polarization, a H ° permeation cell type by Devanathan and Stachurski, and the bacteria Desulfovibrio desuifuricans ssp. desulfuricans. The permeation tests were run using a de-aerated sterile culture medium inoculated with 10% D. dexulfuricans at 108 cell/ml. Bacterial growth was evaluated by the serial dilution technique and Scanning Electron Microscopy (SEM) was used to analyze the characteristics of the biofilm formed on the Pd strip. Results indicated bacterial growth in the order of40xl09 CFU/ml at 24 hours in both polarization and non-polarization tests. This shows that these bacteria develop similarly, whether or not they are on a polarized surface as a source of H °, generating H2S as a product of sulfate-dissimilating activity. It was also determined that, without cathodic polarization, there were no conditions for reduction and permeation of the tf generated by H2S dissociation. When cathodic polarization was applied, an increase was observed in the permeation current, which was associated with the maximum enzymatic activity phase of the bacteria.

Key Words: Hydrogen permeation cell, cathodic depolarization mechanism, Sulfate- Reducing Bacteria, Microbiologically Induced Corrosion, cathodic polarization.
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