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00126 IDENTIFICATION AND CHARACTERIZATION OF SULFATE-REDUCING BACTERIA INVOLVED IN MICROBIALLY INFLUENCED CORROSION IN OIL FIELDS

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Product Number: 51300-00126-SG
ISBN: 00126 2000 CP
Author: M. Nemati and G. Voordouw
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Sulfate-reducing bacteria (SRB) are thought to be involved in microbially influenced corrosion (MIC) of oil field pipelines and equipment. Analysis of microbial communities on corrosion coupons installed in production facilities at Wainwright and Wildmere, two oil fields in Alberta, was done by reverse sample genome probing (RSGP) a DNA hybridization assay in which multiple bacteria can be tracked simultaneously. RSGP indicated dominance of SRB in the microbial communities present on 10 of 15 coupon samples. Of these Desulfovibrio spp. Lac6 and Eth3 were found to be resistant to cocodiamine biocides used in these fields, suggesting that biocide addition could be of limited use for corrosion prevention. Desulfovibrio sp. Lac6 was inhibited by nitrite, and by nitrate if a nitrate-reducing, sulfide-oxidizing bacterium was also added. Addition of nitrite or nitrate thus offer alternative ways to contain SRB, although their effect on corrosion rates have not been extensively studied.
Sulfate-reducing bacteria (SRB) are thought to be involved in microbially influenced corrosion (MIC) of oil field pipelines and equipment. Analysis of microbial communities on corrosion coupons installed in production facilities at Wainwright and Wildmere, two oil fields in Alberta, was done by reverse sample genome probing (RSGP) a DNA hybridization assay in which multiple bacteria can be tracked simultaneously. RSGP indicated dominance of SRB in the microbial communities present on 10 of 15 coupon samples. Of these Desulfovibrio spp. Lac6 and Eth3 were found to be resistant to cocodiamine biocides used in these fields, suggesting that biocide addition could be of limited use for corrosion prevention. Desulfovibrio sp. Lac6 was inhibited by nitrite, and by nitrate if a nitrate-reducing, sulfide-oxidizing bacterium was also added. Addition of nitrite or nitrate thus offer alternative ways to contain SRB, although their effect on corrosion rates have not been extensively studied.
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