Non-oxidizing biocides are commonly used for eradicating microorganisms preventing corrosion and plugging problems and assuring the quality of water for being used in the water injection plant. However such biocides are very expensive and in some cases they are not completely efficient on everywhere. As a result it is necessary to evaluate their efficiency against sessile bacteria before using them in a specific system. Hence this study shows the procedure used for evaluating the effectiveness of a variety of non-oxidizing biocides for controlling MIC by sessile SRB in a water injection plant of a local oil company. A laboratory technical screening of 15 biocides was completed against sessile SRB on carbon steel surfaces at maximum concentration established by the manufacturer and two hours as contact time resulting the glutaraldehyde from one manufacturer as the best biocide to control MIC by SRB in this water injection.