The work aimed to standardize the initial chemical and microbiological composition of seawater used in hibernation studies, due to the seasonality to which these waters are subject throughout the year. Bioaugmentation vs. biostimulation techniques were tested to increase microbial growth and test seawater with greater biocorrosive potential, obtaining more conservative results. Different seawater samples off the coast of Rio de Janeiro/Brazil were tested to validate the experimental model. Cultivation and molecular biology techniques were used for microbial monitoring. At the same time, the concentration of dissolved oxygen in the standardized fluid was monitored. The biostimulation technique was the most appropriate, maintaining a high bacterial concentration throughout the experiment. Population analysis by metagenomics revealed the presence of bacterial groups that promote biocorrosion in all waters and a change in the population profile over time, revealing the adaptability of certain groups. The oxygen concentration decreased over time reaching ppb levels.