Carbon nanotubes with their unique combination of electrical optical mechanical and electrochemical properties have attracted considerable attention for sensor applications. In this work an amperometric biosensor was constructed using carbon nanotubes and conducting polymer for the detection of sulfide. The polymer functionalized carbon nanotubes provided an excellent sensing platform that has large active surface area high sensitivity and fast response. The anti-interference properties of the sensor under field operation condition of pipelines were also investigated. The sensor developed can be used for detection of biogenic sulfide resulting from sulfate-reducing bacteria (SRB) activities and thus working for the monitoring of microbiologically influenced corrosion (MIC) of pipelines. The results paved the way for construction of high-performance biosensors for fast and reliable monitoring of environmentally hazardous sulfide as well as MIC.