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10206 Effect of Water Content on the Growth of SRB in Crude Oil

Picture for 10206 Effect of Water Content on the Growth of SRB in Crude Oil
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Product Number: 51300-10206-SG
ISBN: 10206 2010 CP
Author: Bente-Lise Lillebo, Egil Sunde, Terje Torsvik and Hans Kristian Hornnes
Publication Date: 2010
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Growth of sulfate reducing bacteria (SRB) in crude oil, with variable amounts of enrichment medium was studied. Gullfaks, Snorre and Grane crude oil were used as carbon and energy source. In Gullfaks crude oil anaerobic cultures with 2 to 95 % v/v brine were inoculated with a mixed culture of SRB and incubated at 30 C for 4 weeks. The cultures were amended with volatile fatty acids (VFA) to enhance growth of SRB. Cultures were analyzed for growth of SRB and H2S production. Growth of SRB was observed in cultures with 6 % water and more, and H2S production was observed in cultures with 10 % water and more. In cultures without VFA H2S production was not observed. In Snorre and Grane crude oil anaerobic cultures with 2 to 10 % v/v enrichment medium were inoculated with a mixed culture of SRB and incubated at 30 C for 4 weeks. Half of the cultures were amended with VFA to enhance growth of SRB; the other half contained only crude oil as carbon and energy source. In cultures grown on crude oil from Snorre, with or without VFA, no growth of SRB or sulfide production was observed. In contrast, growth of SRB and H2S production was observed in all cultures with crude oil from Grane, regardless the water amount and whether acids were added or not. Chemical analysis of the crude oil showed major differences in oil composition with regards to the lighter oil components. Snorre oil contained 23 % Wt of oil compounds lighter than C10, while Gullfaks and Grane oil contained 9.7 and 2.9 % Wt respectively. The light oil fraction also contains the most water soluble components, including benzene and toluene known to be toxic to many bacteria, including SRB. Since a typical export specification for crude oil is less than 0.5 % water, the results from Gullfaks and Snorre (light crude oil) showed that growth of SRB in transport pipelines was strongly inhibited under these conditions. In cultures with crude oil from Grane, a heavier crude oil, growth of SRB was observed even at 2 % water content.

Keywords: Crude oil, Gullfaks, Snorre, Grane, water content, anaerobic growth, SRB, H2S, chemical oil analysis
Growth of sulfate reducing bacteria (SRB) in crude oil, with variable amounts of enrichment medium was studied. Gullfaks, Snorre and Grane crude oil were used as carbon and energy source. In Gullfaks crude oil anaerobic cultures with 2 to 95 % v/v brine were inoculated with a mixed culture of SRB and incubated at 30 C for 4 weeks. The cultures were amended with volatile fatty acids (VFA) to enhance growth of SRB. Cultures were analyzed for growth of SRB and H2S production. Growth of SRB was observed in cultures with 6 % water and more, and H2S production was observed in cultures with 10 % water and more. In cultures without VFA H2S production was not observed. In Snorre and Grane crude oil anaerobic cultures with 2 to 10 % v/v enrichment medium were inoculated with a mixed culture of SRB and incubated at 30 C for 4 weeks. Half of the cultures were amended with VFA to enhance growth of SRB; the other half contained only crude oil as carbon and energy source. In cultures grown on crude oil from Snorre, with or without VFA, no growth of SRB or sulfide production was observed. In contrast, growth of SRB and H2S production was observed in all cultures with crude oil from Grane, regardless the water amount and whether acids were added or not. Chemical analysis of the crude oil showed major differences in oil composition with regards to the lighter oil components. Snorre oil contained 23 % Wt of oil compounds lighter than C10, while Gullfaks and Grane oil contained 9.7 and 2.9 % Wt respectively. The light oil fraction also contains the most water soluble components, including benzene and toluene known to be toxic to many bacteria, including SRB. Since a typical export specification for crude oil is less than 0.5 % water, the results from Gullfaks and Snorre (light crude oil) showed that growth of SRB in transport pipelines was strongly inhibited under these conditions. In cultures with crude oil from Grane, a heavier crude oil, growth of SRB was observed even at 2 % water content.

Keywords: Crude oil, Gullfaks, Snorre, Grane, water content, anaerobic growth, SRB, H2S, chemical oil analysis
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