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03554 Comparison of the Microbial Community Composition at Yucca Mountain and Laboratory Test Nuclear Repository Environments

Picture for 03556 Comparison of the Microbial Community
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Product Number: 51300-03556-SG
ISBN: 03556 2003 CP
Author: Joanne Horn, Celena Carrillo, and Victoria Dias
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The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain (YM), NV was determined. Microbial growth from collected rock was detected using simulated ground water as a growth medium, with or without amendment of a carbon source. Grown isolates were identified by 16S ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis of the microbial community located at the proposed nuclear waste repository site was performed using environmental DNA isolation and subsequent identification of amplified 16S rDNA genes. Concurrently, a series of corrosion testing tanks that simulate the evolution of anticipated environmental conditions within the proposed repository have been subjected to the same type of analyses. KEYWORDS MIC; Yucca Mountain; bacterial community profile; biodiversity; nuclear waste
The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain (YM), NV was determined. Microbial growth from collected rock was detected using simulated ground water as a growth medium, with or without amendment of a carbon source. Grown isolates were identified by 16S ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis of the microbial community located at the proposed nuclear waste repository site was performed using environmental DNA isolation and subsequent identification of amplified 16S rDNA genes. Concurrently, a series of corrosion testing tanks that simulate the evolution of anticipated environmental conditions within the proposed repository have been subjected to the same type of analyses. KEYWORDS MIC; Yucca Mountain; bacterial community profile; biodiversity; nuclear waste
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