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01246 Study of Internal MIC in Pipelines of Sour Gas, Mixed with Formation Waters

Picture for 01246 Study of Internal MIC in Pipelines
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Product Number: 51300-01246-SG
ISBN: 01246 2001 CP
Author: M. L. Mora-Mendoza, R. Garcia-Esquivel , A.A. Padilla-Viveros , L. Martinez a, M. Martinez-Bautista,
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Microorganisms isolated from sour gas transmission pipelines from/to offshore platforms located in Mexico, have been associated with localized corrosion process. The microorganisms, isolated under an oxygen-free atmosphere, grew in a culture media rich in lactate/sulfate and reduced sulfate to hydrogen sulfide. When carbon steel was exposed to their activity, severe corrosion rates were measured and pitting damage was observed on sample surfaces. The 16S rRNA sequential analysis procedure showed that both samples are identified as Citrobacter amalonaticus. At present, these bacteria have not been associated with MIC in transmission hydrocarbon pipelines. Bacterial membrane proteins were analyzed by SDS-PAGE. The Total Membrane Proteins (TMP), Outer Membrane Proteins (OMP) and Adhesion Proteins (AP) profiles were repetitive for the 2 strains analyzed, even though they differed from each other in origin, growth patterns, biofilm formation capacity and corrosion rates. Two proteins of permanent adhesion were detected (one at 50 kDa and the other one at 25 kDa). Both are OMP that promote adhesion from the bacterial cells to the metallic surface.
Microorganisms isolated from sour gas transmission pipelines from/to offshore platforms located in Mexico, have been associated with localized corrosion process. The microorganisms, isolated under an oxygen-free atmosphere, grew in a culture media rich in lactate/sulfate and reduced sulfate to hydrogen sulfide. When carbon steel was exposed to their activity, severe corrosion rates were measured and pitting damage was observed on sample surfaces. The 16S rRNA sequential analysis procedure showed that both samples are identified as Citrobacter amalonaticus. At present, these bacteria have not been associated with MIC in transmission hydrocarbon pipelines. Bacterial membrane proteins were analyzed by SDS-PAGE. The Total Membrane Proteins (TMP), Outer Membrane Proteins (OMP) and Adhesion Proteins (AP) profiles were repetitive for the 2 strains analyzed, even though they differed from each other in origin, growth patterns, biofilm formation capacity and corrosion rates. Two proteins of permanent adhesion were detected (one at 50 kDa and the other one at 25 kDa). Both are OMP that promote adhesion from the bacterial cells to the metallic surface.
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