Products tagged with 'primers'

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Development of Molecular Probe for Iron Reducing and Thiosulfate Reducing Bacteria

Product Number: 51324-20885-SG

Author: Tekle Fida; Luke Austin; Scott Leleika; Taylor Rambo; Karen Crippen

Publication Date: 2024

$40.00

Microbiologically Influenced Corrosion (MIC) activities are mostly monitored by using canonical culture-based methods. However, these methods detect only culturable bacteria or require months of incubation to get results. To overcome this, molecular detection methods were developed for many of the MIC-causing microbes but were not available for Iron Reducing Bacteria (IRB) and Thiosulfate Reducing Bacteria (TRB). IRB and TRB are among the dominant group of microbes involved in corrosion and souring oil and gas infrastructures. In the present study, molecular probes were developed for IRB and TRB by targeting functional genes implicated in iron and thiosulfate reduction, respectively. Candidate gene sequences were retrieved from genome repository databases of pure isolates or metagenomic sequences. The sequences were aligned using bioinformatic software and primer-based probes were developed from conserved regions. Specificity and efficiency of the primers were validated by amplifying the sequences of target and non-target microbes using Quantitative Polymerase Chain Reaction (qPCR) methods. The probes successfully detected candidate microbes in biocorrosion samples collected from various gas processing facilities. The identified primer-based probes will help oil and gas industries to monitor MIC activities and develop timely management strategies to prevent undesirable microbial activities while improving system integrity and safety concerns.

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Two Coat Inorganic Coatings System for Corrosion Resistance and Long-Life

Product Number: 51324-21092-SG

Author: Kristen Blankenship; James Lawson

Publication Date: 2024

$40.00

Extending service life of an offshore wind tower brings value to the owner and has the added benefit of reducing environmental impact. Arguably the biggest threat to service- life is degradation. When constructing with steel, corrosion is the threat to mitigate. Coatings formulated with zinc dust have been the primary strategy for protection. Zinc dust incorporated into silicate resins is considered an inorganic zinc coating. In recent years silicate finishes made without zinc have entered the market to create a two- coat inorganic system offering unmatched corrosion protection in a finish with various color options. The silicate resin is low carbon and won’t contribute to microplastic accumulation in the ocean. This paper will explain what a two- coat inorganic corrosion resistant system is, how it works, what it looks like and most importantly how it extends the life- cycle of wind towers by inhibiting corrosion for decades.

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Use of Pre-Construction Primers in Marine Construction

Product Number: 41215-876-SG

Author: J. Peter Ault

Publication Date: 2015

$20.00

Pre-construction primers (also known as shop primers or pre-fabrication primers) are very thin films applied to blast cleaned steel plates and shapes to provide preservation of the blasted steel during the shipbuilding or construction process. SSPC is developing an industry guide document containing information regarding the use of pre-construction primers (PCP) on structural steel in shipbuilding.

Products tagged with 'primers'

Development of Molecular Probe for Iron Reducing and Thiosulfate Reducing Bacteria

Two Coat Inorganic Coatings System for Corrosion Resistance and Long-Life

Use of Pre-Construction Primers in Marine Construction

Association for Materials Protection and Performance