Low frequency cyclic stresses are experienced by the materials in a number of applications. Effect of low frequency cyclic stresses on stress corrosion cracking (SCC) of duplex stainless steel (DSS) 2205 was investigated in this study. Previous work has suggested that the two phases (austenite and ferrite) of DSSs have different residual stresses and different SCC susceptibilities in acidic chloride and caustic white liquor environments. To understand the effect of low frequency fatigue on SCC initiation and propagation tests were performed on the as-received and work-hardened DSS 2205 in two different environments where SCC could occur: 26 wt% NaCl at room temperature and white liquor at 170 °C. Test results on as-received material showed that cyclic stresses facilitate crack initiation. Inclusions and austenite phase were the preferential sites for cracks to initiate in chloride and WL environments respectively. The effects of fatigue parameters like stress ratio and maximum stress level of the cyclic stresses were also characterized. Test results on work-hardened material illustrated that work hardening enhanced SCC of DSS 2205. The crack initiation sites of the work-hardened DSS 2205 in these two environments were associated with microstructural features such as pits and precipitates.