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Combined Effects of Microbes and Nitrate on SRB Growth Souring and Corrosion

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A stationary testing method and a porous media flow reactor method were used for these studies. The effect of different NRB, including NO2NRB and sulfide-oxidizing NRB (SONRB) on corrosion of carbon steel beads in the presence and absence of nitrate was also investigated using porous media flow reactors.

Product Number: 51317--9425-SG
ISBN: 9425 2017 CP
Author: Bei Yin
Publication Date: 2017
Industry: Science of Corrosion
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Controlling reservoir souring is critical to successful production and asset protection in hydrocarbon recovery. Nitrate injection has been widely used in water flooding as a primary barrier for controlling biologically generated sulfide in oil reservoirs. Souring control via nitrate implementation is dynamically affected by the interactions between nitrate-reducing bacteria (NRB) and sulfate-reducing bacteria (SRB). Resultantly it is important to understand how the community profile and the availability of electron acceptor/donor in the environment controls the effectiveness of nitrate on SRB growth sulfide levels and corrosion.In laboratory studies the efficacy of nitrate treatments were evaluated on SRB (with or without nitrate-reducing capability) combined with various NRB including denitrifiers (reduce nitrate to N2) nitrite-producing NRB and sulfide-oxidizing NRB in the presence of different concentrations of electron acceptor/donor of these bacteria. Stationary and porous media flow reactor methods were used for these studies and sulfide concentrations and viable SRB counts were measured during and or after each treatment. The effect of microbial composition on corrosion of carbon steel beads in the presence and absence of nitrate was also investigated using porous media flow bioreactors. It was found that the effectiveness of nitrate on SRB growth sulfide level and metal corrosion can be dramatically affected by the types of SRB and NRB present in the testing system. The corrosion studies illustrate that sulfide control via nitrate treatment doesn’t necessarily reduce corrosion risks. Microbial activities and metabolites other than sulfide can also play important roles on the corrosion results. These studies indicate that the effectiveness of nitrate treatment in the field can be impacted by both biotic and abiotic factors which need to be considered when designing nitrate treatment strategies.

Key words: Souring, nitrate, nitrate-reducing bacteria, sulfate-reducing bacteria

Controlling reservoir souring is critical to successful production and asset protection in hydrocarbon recovery. Nitrate injection has been widely used in water flooding as a primary barrier for controlling biologically generated sulfide in oil reservoirs. Souring control via nitrate implementation is dynamically affected by the interactions between nitrate-reducing bacteria (NRB) and sulfate-reducing bacteria (SRB). Resultantly it is important to understand how the community profile and the availability of electron acceptor/donor in the environment controls the effectiveness of nitrate on SRB growth sulfide levels and corrosion.In laboratory studies the efficacy of nitrate treatments were evaluated on SRB (with or without nitrate-reducing capability) combined with various NRB including denitrifiers (reduce nitrate to N2) nitrite-producing NRB and sulfide-oxidizing NRB in the presence of different concentrations of electron acceptor/donor of these bacteria. Stationary and porous media flow reactor methods were used for these studies and sulfide concentrations and viable SRB counts were measured during and or after each treatment. The effect of microbial composition on corrosion of carbon steel beads in the presence and absence of nitrate was also investigated using porous media flow bioreactors. It was found that the effectiveness of nitrate on SRB growth sulfide level and metal corrosion can be dramatically affected by the types of SRB and NRB present in the testing system. The corrosion studies illustrate that sulfide control via nitrate treatment doesn’t necessarily reduce corrosion risks. Microbial activities and metabolites other than sulfide can also play important roles on the corrosion results. These studies indicate that the effectiveness of nitrate treatment in the field can be impacted by both biotic and abiotic factors which need to be considered when designing nitrate treatment strategies.

Key words: Souring, nitrate, nitrate-reducing bacteria, sulfate-reducing bacteria

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